Anther Culture Efficiency According to Plating Method in Naked Barley

Influence of anther pretreatment on the efficiency of androgenesis in barley.

The change in the developmental pathway of microspores from gametophytic to sporophytic is induced by stress during pretreatment of spikes and anthers. In our experiments, anther culture of three barley cultivars was tested with regard to the effect of chilling at 4 degrees C for 28 days, starvation in 0.3 M mannitol solution for 4 days, and a combination of both methods. Chilling was shown to ...

New findings in strawberry (Fragaria × ananassa Duch.) anther culture

In this research, some independent experiments were carried out for investigating the effects of microspore developmental stage, bud pretreatments (cold, heat and Ca), and embryo induction medium on anther culture of some strawberry cultivars. The results showed that the percentages of responsive and embryogenic anthers in cultivar Paros in liquid medium were influenced not only by different de...

Anther culture of iranian wheat germplasm using different media

Colchicine induced embryogenesis and doubled haploid production in maize (Zea mays L.) anther culture

This study involves in vitro androgenesis of <sp...

Tissue Culture and Quantification of Individual-level Resistance to Anther-smut Disease in Silene Vulgaris

Resistance to systemic diseases is often difficult to measure quantitatively because individuals can be scored only as either diseased or healthy. A technique is described for cloning individuals of Silene vulgaris plants using the tissue culture of seedling meristems. Inoculation of such clonally replicated genotypes within two families shows that segregation of resistance to anther-smut disea...